Current methods used for isolating Salmonella from feed and feed ingredients have been derived from human food methods with minimum consideration to differences in matrix compositions, background microflora or the stress status of the Salmonella, said Mark Berrang et al.
Salmonella can contaminate feed production facilities and feed ingredients (Boyer et al., 1962; Maciorowski et al., 2007; Jones, 2011). In some instances, human outbreaks of Salmonella infections have been traced back to Salmonella-contaminated feed given to poultry (Pennington et al., 1968; Crump et al., 2002).
While pre-enrichment media are designed to revive stressed or injured Salmonella for detection in later analytical steps, prior research has demonstrated that when analyzing feed and feed ingredients, a decrease in the pH of the pre-enrichment media occurs during the incubation period creating acidic conditions, said those researchers.
"Exposure of Salmonella to acidic conditions can result in cell death, cell injury and biochemical damage. This reduces the ability to detect Salmonella and could produce false negative results.”
The team reported that pre-enrichment of feed and feed ingredients in tris phosphate carbonate (TPC) appears to lessen these challenges. The TPC was developed to maintain a near neutral pH during incubation of feed but its efficacy for use in a wide variety of feed and ingredients had not been confirmed until now.
Feed is a complex matrix containing a wide variety of ingredients ranging from cereal grains/protein meals to enzymes, probiotics, essential oils, acidifiers and others. The impact of these components on incubation pH during pre-enrichment is unknown, they said. Feed can also contain a wide diversity of background microflora which may impact the media pH during incubation (Koyuncu and Haggblom 2009), noted the team.
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October 26, 2020 at 09:36PM
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Tool to make Salmonella detection in poultry feed less challenging - FeedNavigator.com
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